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bglii restriction sites  (TaKaRa)


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    Structured Review

    TaKaRa bglii restriction sites
    Bglii Restriction Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 3916 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bglii+restriction+site/Bgl+II/pm40102394-370-32-56
    Average 97 stars, based on 3916 article reviews
    bglii restriction sites - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: New BB0108, BB0126, BB0298, BB0323, and BB0689 Chromosomally Encoded Recombinant Proteins of Borrelia burgdorferi sensu lato for Serodiagnosis of Lyme Disease
    Article Snippet: .. The PCR products were inserted into the BglII restriction site of pUET1 expression plasmid using In-Fusion ® HD Cloning Kit (Takara Bio USA, Inc., Mountain View, CA, USA) ( ) [ ]. ..

    Expressing:

    Article Title: New BB0108, BB0126, BB0298, BB0323, and BB0689 Chromosomally Encoded Recombinant Proteins of Borrelia burgdorferi sensu lato for Serodiagnosis of Lyme Disease
    Article Snippet: .. The PCR products were inserted into the BglII restriction site of pUET1 expression plasmid using In-Fusion ® HD Cloning Kit (Takara Bio USA, Inc., Mountain View, CA, USA) ( ) [ ]. ..

    Plasmid Preparation:

    Article Title: New BB0108, BB0126, BB0298, BB0323, and BB0689 Chromosomally Encoded Recombinant Proteins of Borrelia burgdorferi sensu lato for Serodiagnosis of Lyme Disease
    Article Snippet: .. The PCR products were inserted into the BglII restriction site of pUET1 expression plasmid using In-Fusion ® HD Cloning Kit (Takara Bio USA, Inc., Mountain View, CA, USA) ( ) [ ]. ..

    Article Title: Tcf3 and Tcf4 are essential for long-term homeostasis of skin epithelia
    Article Snippet: The PCR product was digested with BamH1 and ligated to a Krt14 promoter cassette that was digested with BamH1 and phosphatase treated with shrimp alkaline phosphatase (SAP; Roche). .. After its sequences were verified, the Krt14-Tcf4 template was used to amplify the Tcf4 gene with a BglII restriction site introduced in its 5′ end so as to be in-frame when ligated into the pCMVmyc vector (Clontech). ..

    Article Title: PINK1 protects against cell death induced by mitochondrial depolarization, by phosphorylating Bcl-xL and impairing its pro-apoptotic cleavage.
    Article Snippet: .. The pEGFP–Bcl-xL vector used in immunofluorescence experiments was generated by inserting full-length Bcl-xL into the BglII restriction site of pEGFPC1 (Clontech, Mountain View, CA, USA). .. To assess the N-terminal cleavage of Bcl-xL, a construct tagged at the C-terminus with the Myc epitope (pCMV6-Bcl-xL) was purchased by OriGene (Rockville, MD, USA).

    Article Title: Crym -positive striatal astrocytes gate perseverative behaviour
    Article Snippet: .. The three U6-sgRNAs were then combined in the same vector using a BglII restriction site and the In-Fusion cloning kit (Takara Bio 638943). ..

    Article Title: PINK1 protects against cell death induced by mitochondrial depolarization, by phosphorylating Bcl-xL and impairing its pro-apoptotic cleavage
    Article Snippet: .. The pEGFP–Bcl-xL vector used in immunofluorescence experiments was generated by inserting full-length Bcl-xL into the BglII restriction site of pEGFP-C1 (Clontech, Mountain View, CA, USA). .. To assess the N-terminal cleavage of Bcl-xL, a construct tagged at the C-terminus with the Myc epitope (pCMV6-Bcl-xL) was purchased by OriGene (Rockville, MD, USA).

    Cloning:

    Article Title: New BB0108, BB0126, BB0298, BB0323, and BB0689 Chromosomally Encoded Recombinant Proteins of Borrelia burgdorferi sensu lato for Serodiagnosis of Lyme Disease
    Article Snippet: .. The PCR products were inserted into the BglII restriction site of pUET1 expression plasmid using In-Fusion ® HD Cloning Kit (Takara Bio USA, Inc., Mountain View, CA, USA) ( ) [ ]. ..

    Article Title: Crym -positive striatal astrocytes gate perseverative behaviour
    Article Snippet: .. The three U6-sgRNAs were then combined in the same vector using a BglII restriction site and the In-Fusion cloning kit (Takara Bio 638943). ..

    Immunofluorescence:

    Article Title: PINK1 protects against cell death induced by mitochondrial depolarization, by phosphorylating Bcl-xL and impairing its pro-apoptotic cleavage.
    Article Snippet: .. The pEGFP–Bcl-xL vector used in immunofluorescence experiments was generated by inserting full-length Bcl-xL into the BglII restriction site of pEGFPC1 (Clontech, Mountain View, CA, USA). .. To assess the N-terminal cleavage of Bcl-xL, a construct tagged at the C-terminus with the Myc epitope (pCMV6-Bcl-xL) was purchased by OriGene (Rockville, MD, USA).

    Article Title: PINK1 protects against cell death induced by mitochondrial depolarization, by phosphorylating Bcl-xL and impairing its pro-apoptotic cleavage
    Article Snippet: .. The pEGFP–Bcl-xL vector used in immunofluorescence experiments was generated by inserting full-length Bcl-xL into the BglII restriction site of pEGFP-C1 (Clontech, Mountain View, CA, USA). .. To assess the N-terminal cleavage of Bcl-xL, a construct tagged at the C-terminus with the Myc epitope (pCMV6-Bcl-xL) was purchased by OriGene (Rockville, MD, USA).

    Generated:

    Article Title: PINK1 protects against cell death induced by mitochondrial depolarization, by phosphorylating Bcl-xL and impairing its pro-apoptotic cleavage.
    Article Snippet: .. The pEGFP–Bcl-xL vector used in immunofluorescence experiments was generated by inserting full-length Bcl-xL into the BglII restriction site of pEGFPC1 (Clontech, Mountain View, CA, USA). .. To assess the N-terminal cleavage of Bcl-xL, a construct tagged at the C-terminus with the Myc epitope (pCMV6-Bcl-xL) was purchased by OriGene (Rockville, MD, USA).

    Article Title: PINK1 protects against cell death induced by mitochondrial depolarization, by phosphorylating Bcl-xL and impairing its pro-apoptotic cleavage
    Article Snippet: .. The pEGFP–Bcl-xL vector used in immunofluorescence experiments was generated by inserting full-length Bcl-xL into the BglII restriction site of pEGFP-C1 (Clontech, Mountain View, CA, USA). .. To assess the N-terminal cleavage of Bcl-xL, a construct tagged at the C-terminus with the Myc epitope (pCMV6-Bcl-xL) was purchased by OriGene (Rockville, MD, USA).



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